Development of the anti-AIDS drug using aptamer-siRNA

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460146
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Drug development chemistry
• Research Institution: Nagasaki University
• Principal Investigator: KABASHIMA Tsutomu 長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (20274673)
• Co-Investigator(Renkei-kenkyūsha): KAI Masaaki 長崎大学, 医歯薬学総合研究科(薬学系), 教授 (00160953) ; SHIBATA Takayuki 長崎大学, 医歯薬学総合研究科(薬学系), 助教 (10448491)
• Research Collaborator: SHIMAMURA Ryosuke ; OOSHIMA Sumika ; ADACHI Anna ; JINNOUCHI Nobutoshi ; TANOUE Naomi ; KAWASHIMA Kaori ; SOMIYA Miwako ; YIN Sheng ; Ejupi Valon
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: エイズ / HIV / アプタマー / siRNA / プロテアーゼ / CD4 / 変異 / 薬剤耐性

Outline of Final Research Achievements

Novel nucleic acid molecule that combined DNA aptamer and siRNA (DNA aptamer-siRNA: DAS) was developed. DNA aptamer of DAS recognizes the CD4, which is essential for HIV infection, and siRNA of DAS represses the expression of HIV protease. Anti-HIV effects of DAS were examined. DAS was selectively taken into CD4+ T cell, and reduced the expression of HIV protease. Furthermore, DAS was relatively stable in serum. These results indicated that the possibility as the anti-HIV drug of DAS.

Free Research Field

薬学