Establishment of high expression system for therapeutic protein production using human cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460167
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Drug development chemistry
• Research Institution: Kitakyushu National College of Technology
• Principal Investigator: Inoue Yuichi 北九州工業高等専門学校, 生産デザイン工学科, 准教授 (20284911)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ヒト細胞株 / 医療用タンパク質 / GMP

Outline of Final Research Achievements

To establish new GMP-compliant human cell lines for therapeutic protein production, we determined the conditions for making immortalized cells from human peripheral blood mononuclear cells in a non-GMP environment. Then, we improved them for a GMP environment and succeeded in obtaining human B lymphoid clones that could be cultured for a long time. The obtained clones might be mainly suitable for secreted protein production because mature B lymphocytes have the extensive endoplasmic reticulum. Further study on serum-free cell culture and cell protein productivity enhancement will lead to a practical application of therapeutic protein production using human cells.

Free Research Field

農学