2016 Fiscal Year Final Research Report
Establishment of high expression system for therapeutic protein production using human cells
Project/Area Number |
26460167
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Drug development chemistry
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Research Institution | Kitakyushu National College of Technology |
Principal Investigator |
Inoue Yuichi 北九州工業高等専門学校, 生産デザイン工学科, 准教授 (20284911)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Keywords | ヒト細胞株 / 医療用タンパク質 / GMP |
Outline of Final Research Achievements |
To establish new GMP-compliant human cell lines for therapeutic protein production, we determined the conditions for making immortalized cells from human peripheral blood mononuclear cells in a non-GMP environment. Then, we improved them for a GMP environment and succeeded in obtaining human B lymphoid clones that could be cultured for a long time. The obtained clones might be mainly suitable for secreted protein production because mature B lymphocytes have the extensive endoplasmic reticulum. Further study on serum-free cell culture and cell protein productivity enhancement will lead to a practical application of therapeutic protein production using human cells.
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Free Research Field |
農学
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