Generation of vascular smooth muscle specific Ubiad1 gene knockout mice and phenotype analysis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460180
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Environmental and hygienic pharmacy
• Research Institution: Osaka Shoin Women's University (2015-2016); Kobe Pharmaceutical University (2014)
• Principal Investigator: Tsugawa Naoko 大阪樟蔭女子大学, 健康栄養学部, 教授 (30207352)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: ビタミンK / UBIAD1 / 血管特異的遺伝子欠損 / 血管平滑筋 / Transgelin-Cre

Outline of Final Research Achievements

UbiA prenyltransferase domain containing 1 (UBIAD1) is a novel vitamin K2 biosynthetic enzyme. To investigate the function of UBIAD1 in vascular smooth muscle cells, we attempted to generate vascular specific conditional Ubiad1 knockout mice (Ubiad1-V-KO). Ubiad1-V-KO was generated with conditional deletion of Ubiad1 gene in cells expressing the smooth muscle cell-specific protein SM22α. Ubiad1-V-KO were born according to Mendel's theory of heredity, however all Ubiad1-V-KO were died with pleural effusion at 6-8 weeks-old. Also, we confirmed that abnormality of aortic elastic fiber, ventricular myocardial hypoplasia, hypertension and decrease of heart rate in Ubiad1-V-KO.

Free Research Field

衛生化学