The development of novel biomarker for arteriosclerosis using circulating miRNAs.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460203
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Nagoya City University
• Principal Investigator: Iwaki Soihiro 名古屋市立大学, 大学院薬学研究科, 講師 (60399962)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: miRNA / バイオマーカー / 動脈硬化

Outline of Final Research Achievements

To identify the novel miRNA markers for arteriosclerosis, we screened total RNA samples derived from patient's plasma using a microarray platform. We found that several circulating miRNAs were differentially expressed. We also demonstrated that expression of miRNAs was altered by various cytokine that induced the inflammation state in the human umbilical cord vein endothelial cells (HUVECs). Furthermore, we investigated the optimal RNA extraction method of miRNA from plasma samples for quantitative PCR. These results suggest that circulating miRNAs are available as a novel biomarker to assess the arteriosclerosis.

Free Research Field

分子生物学