2016 Fiscal Year Final Research Report
Determination of conjugated steroids in dried urine spots by means of liquid chromatography-mass spectrometry
| Project/Area Number |
26460211
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Kindai University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 乾燥ろ紙尿 / LC/MS / テトラヒドロコルチコステロイド / オキシステロール / グルクロン酸抱合体 / 硫酸抱合体 |
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| Outline of Final Research Achievements |
Profile determination methods for oxysterols sulfate and tetrahydrocorticosteroids glucuronides (THC-G) and/or these sulfates in urine samples by means of LC/MS/MS were developed. The method was applicable to quantitative analysis of THC-G in dried urine spots (DUS) sample. The method was well validated, and the concentration of THC-G in DUS were well correlated with those in liquid urine. DUS samples provides convenient and safe transportation, storage and handling of sample. DUS samples would be a useful alternative for clinical screening tests of endocrine disorders, particularly, when the sample needs to be transported over long distances.
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| Free Research Field |
臨床分析化学
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