Analysis of Caspr in glioma invasion

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460222
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Kagoshima University
• Principal Investigator: Takeda Yasuo 鹿児島大学, 医歯学域附属病院, 教授 (60245462)
• Research Collaborator: FUTAGAWA TOSHITAKA 鹿児島大学, 病院, 薬剤師 ; TAKAHAMA KAZUHIRO 鹿児島大学, 病院, 薬剤師 ; MASUDA SHOGO 鹿児島大学, 病院, 薬剤師
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: Caspr1 / 膠芽腫 / 移動・浸潤 / JNK / MMP-9

Outline of Final Research Achievements

Caspr family molecules are transmembrane glycoproteins of neurexin superfamily which play a role for myelination of axons in brain. Recently, it has been reported about functions of Caspr family molecules in cancer cells. However, it is unclear about roles of Caspr molecules in cancer cells. In this study, we surveyed expression of Caspr in human glioblastoma cell-lines, and found expression of Caspr1. Secondly, we investigated relation of Caspr1 expression levels and cells proliferation, migration, invasion. In the results, suppression of Caspr1 by treatment with Caspr1-targeted siRNA promoted T98G glioblastoma cells invasion, migration and increased MMP-9 expression levels. Moreover, knockdown of Caspr1 increased JNK phosphorylation, and JNK inhibitor blocked MMP-9 up-regulation and cells invasion, migration by knockdown of Caspr1. Thus, these results suggest that Caspr1 negatively regulates for T98G glioblastoma cells invasion and migration via down-regulation of JNK pathway.

Free Research Field

医歯薬学