2016 Fiscal Year Final Research Report
Analysis on nAChRs in the somatosensory system
| Project/Area Number |
26460251
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | アセチルコリン |
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| Outline of Final Research Achievements |
In the present study, I investigated the cellular expression of nicotinic acetylcholine receptor (nAChR) subunits in the mouse somatosensory system. I made specific riboprobes for a2, a4, a7 and b2 nAChR, and found that their expression levels in the somatosensory system were below the detection threshold. Then I took another approach to localize a7 nAChR by assessing its interacting protein TMEM35 (NACHO). I raised a specific antibody and riboprobe for NACHO and found that NACHO was more highly expressed in glutamatergic neurons than GABAergic neurons in the trigeminal nuclei. On the contrary, it was much higher in GABAergic neurons than glutamatergic neurons in the VPM and somatosensory cortex. These results suggest that the expression pattern of a7 nAChR is nearly opposite in the trigeminal nuclei and its upstream regions, thereby playing distinct functions in the somatosensory system.
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| Free Research Field |
神経解剖学
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