2016 Fiscal Year Final Research Report
Glycolysis, but not Mitochondria, responsible for intracellular ATP distribution in cortical area of podocytes
| Project/Area Number |
26460274
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
General anatomy (including histology/embryology)
|
|---|
| Research Institution | Nara Medical University (2016)
Kyoto University (2014-2015) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 腎臓病 / ポドサイト / ミトコンドリア / 解糖系 |
|---|
| Outline of Final Research Achievements |
Differentiated podocytes maintain glomerular physiology and require substantial levels of energy. Mitochondria and glycolysis are two major producers of ATP, but the precise roles of each in podocytes remain unknown. Mitochondria in differentiated podocytes are present in the central part of cell body. However, blocking mitochondria had minor effects on cell shape and migratory ability. In contrast, blocking glycolysis significantly reduced the formation of lamellipodia, a cortical area of these cells, and decreased cell migratory ability. Intracellular ATP distribution in lamellipodia was regulated only by glycolysis. Inhibiting glycolysis resulted in apoptosis, prevented differentiation, and impaired lamellipodia formation while inhibition of mitochondria reduced synaptopodin expression. These data suggest that mitochondria and glycolysis play parallel but distinct roles in differentiated and differentiating podocytes.
|
| Free Research Field |
腎臓病
|
