Connectomics analysis of the synapse in the primary visual cortex layer IV in the ocular dominant plasticity

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460283
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Meiji Pharmaceutical University
• Principal Investigator: Nakadate Kazuhiko 明治薬科大学, 薬学部, 准教授 (80372895)
• Co-Investigator(Renkei-kenkyūsha): IMAMURA Kazuyuki 前橋工科大学, 工学部, 教授 (30203326) ; SAKAKIBARA Shin-ichi 早稲田大学, 人間科学学術院, 教授 (70337369) ; UEDA Shuichi 獨協医科大学, 医学部, 教授 (60150570)
• Research Collaborator: SHIGEMOTO Ryuichi Institute of Science and Technology, 教授
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: コネクトミクス解析 / 視覚野 / シナプス / 眼優位可塑性 / 電子顕微鏡 / 三次元再構築

Outline of Final Research Achievements

To clarify the mechanism of the neural circuit reorganization, we have established connectomics analysis of visual cortex. After monocular deprivation, the neurons in the visual cortex were more received from open eye input. As a result of having immunohistochemically analyzed using c-Fos, FosB and CREB, these activity dependence inducted proteins were resulted to detect the changes of ocular dominance plasticity. Using connectomics analysis, synaptic connections were cyclopedically analyzed in layer IV of visual cortex. As a result, we detected that first the synapses received from deprived eye input were reduced, next the synapses received from opened eye input were increased.

Free Research Field

シナプス可塑性