Novel anti-cancer mechanism of the targeting of the association between receptor-type sodium pump and VSOR channels

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460291
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: University of Toyama
• Principal Investigator: FUJII Takuto 富山大学, 大学院医学薬学研究部(薬学), 助教 (50567980)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 癌 / ナトリウムポンプ / 生理学 / 細胞・組織 / イオンチャネル

Outline of Final Research Achievements

Cardiac glycosides, specific Na,K-ATPase inhibitors, has been suggested to be a valuable anti-cancer drugs. However, the molecular mechanisms for anti-cancer effects of the drugs has not been fully understood. In this study, we have clarified that non-pumping (receptor type) Na,K-ATPase is functionally coupled with volume-sensitive outwardly rectifying (VSOR) anion channels in membrane microdomains of human cancer cells. Submicromolar ouabain activates the cancer cells-specific crosstalk between Na,K-ATPase and VSOR channels in membrane microdomains mediated by NADPH oxidase-dependent reactive oxygen species generation. The activation of VSOR channels elicits anti-proliferative effects in cancer cells.

Free Research Field

分子生理学