2016 Fiscal Year Final Research Report
Effects of synaptotagmin binding on SNARE-mediated fusion and trans-SNARE complex formation
Project/Area Number |
26460297
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General physiology
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Research Institution | Okayama University |
Principal Investigator |
Nishiki Teiichi 岡山大学, 医歯薬学総合研究科, 准教授 (70423340)
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Research Collaborator |
FUJINO Kanako
IWAFUJI Ryota
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Keywords | 神経科学 / 刺激分泌連関 / シナプス小胞 / カルシウムイオン / 開口放出 |
Outline of Final Research Achievements |
SNAREs (syntaxin/SNAP-25/synaptobrevin) and synaptotagmin play central roles during Ca2+-dependent neurotransmitter release. In this study, we have studied the effects of synaptotagmin binding on SNARE-mediated fusion and its complex formation. Synaptotagmin stoichiometrically bound to syntaxin in the liposomes containing t-SNAREs (syntaxin, SNAP-25) and dissociated from it by Ca2+. The fusion between synaptobrevin-liposomes and t-SNARE liposomes was inhibited in the presence of synaptotagmin cytoplasmic fragment. This inhibition was reversed by Ca2+. FRET analysis of trans-SNARE complex formation showed that the assembly of SNARE motifs appears to proceed to +6th layers when synaptotagmin binds syntaxin. These results support the idea where synaptotagmin suppresses SNARE-mediated membrane fusion through its syntaxin binding until Ca2+ influx without inhibiting the full assembly of the trans-SNARE complex.
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Free Research Field |
生理学
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