Activation mechanism of Pannexin 1 channel by using patch-fluorometry

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460300
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General physiology
• Research Institution: Kyushu Nutrition Welfare University (2015-2016); Kyoto Prefectural University of Medicine (2014)
• Principal Investigator: NOMURA Takeshi 九州栄養福祉大学, リハビリテーション学部, 准教授 (10706790)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: パネキシン / ゲーティングキネティクス / 機械受容 / 膜電位 / イオンチャネル

Outline of Final Research Achievements

Pannexin 1 (Panx1) is activated in response to membrane depolarization, mechanical stress, caspase-mediated cleavage of the C-terminus and interaction with activated P2X7 receptor. Panx1 contributes to release of ATP, and act as a‘find-me’ signal that recruits macropharges to apoptic cells. Although the open probability of Panx1 channel increases by membrane depolarization, the detailed single-channel gating kinetics of Panx1 channel is still largely unknown. Here, we investigated the electrophysiological characteristics of mouse Panx1 channel by using patch-clamp technique. The voltage-dependency of single-channel opening and closing rates are dramatically different at hyperpolarized and depolarized potentials in the boundary of the reversal potential. These results suggest that the direction of movement and the quantity of movement per unit time of charge carrier control the voltage-dependent opening and closing rates of Panx1 channel.

Free Research Field

機械受容チャネル