2016 Fiscal Year Final Research Report
Effects of early-life stress on the development of obesity
| Project/Area Number |
26460322
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | オキシトシン / PrRP / 社会的遊び / 社会的ストレス / 摂食 / 肥満 |
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| Outline of Final Research Achievements |
We examined the effects of early-life social stress (deprivation of social play) upon food intake and body weight in adulthood of rats. Social play induced the activation of oxytocin neurons in the paraventricular hypothalamic nucleus. Oxytocin neurons in the paraventricular hypothalamic nucleus were also activated in the observer rats (which observed playing cagemates). Contrary to our expectations, food intake and body weight of chronic social stressed rats were not significantly different from control rats.
We generated two transgenic rat strains that express human diphtheria toxin receptor under the control of the oxytocin promoter and express human interleukin-2 receptor alpha-subunit under the control of the prolactin-releasing peptide (PrRP) promoter. We succeeded in site-specific destruction of oxytocin neurons or PrRP neurons by local injection of diphtheria toxin or immunotoxin in transgenic rats.
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| Free Research Field |
分子神経科学、生理学
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