2016 Fiscal Year Final Research Report
Screening for peptide ligands that activate beta-arrestin biasd cell signaling of orphan GPCRs
| Project/Area Number |
26460333
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YANAGISAWA Masashi 国際統合睡眠医科学研究機構, 教授 (20202369)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | GPCR / Gタンパク質 / β-アレスチン / 生理活性ペプチド |
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| Outline of Final Research Achievements |
Our purpose of this study is to identify endogenouse ligands for orphan GPCRs by newly developed screening systems. We utilized two intracellular signaling pathways, G-proteins and β-arrestin for detection of GPCR activation. To detect β-arrestin signaling, we monitored the ligand activated GPCR-mediated β-arrestin recruitment by using enzymatic complementation of E. coli β-galactosidase, alpha and omega fragments. In terms of detection of G-protein signaling, we generated a new technology that simply monitoring activities of any G-protein alpha subunits leading to minimization of experimental steps. We have optimized these assay systems suitable for high through put screening and being conducting the screening.
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| Free Research Field |
分子薬理学
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