2016 Fiscal Year Final Research Report
Transcriptional regulation of cation channels by iPS factors and the expression deficient diseases
| Project/Area Number |
26460349
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General pharmacology
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | iPS / カチオンチャネル / 転写 |
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| Outline of Final Research Achievements |
We here examined effects of iPS factors, Sox2 and Klf4, on expression of TRP channels in HEK cells where iPS factors were hetelorogously expressed. Sox2 induced the expression of TRPC6, TRPM1, TRPM2, and TRPV2, whereas it reduced the expression of TRPA1. On the other hand, Klf4 induced the marked expression of TRPC5, TRPC6, TRPM2, TRPM4, TRPM6, and TRPV2, whereas it reduced the expression of TRPC1. These results suggest that iPS factors, Sox2 and Klf4, regulate gene-expression of ion channels. In this study, we also demonstrate that differentiation of non-neuronal cell line neuroblastorma to neuron induces functional expression of TRPM3.
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| Free Research Field |
薬効解析学
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