Understanding of the molecular mechanisms of hematopoietic stem cell heterogeneity by quiescence regulatory factors

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460361
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Kanazawa University
• Principal Investigator: Ueno Masaya 金沢大学, がん進展制御研究所, 助教 (20334766)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 幹細胞 / 白血病 / 不均一性 / 治療抵抗性

Outline of Final Research Achievements

Recent studies have revealed that hematopoietic stem cells (HSCs) form heterogeneous population with extremely diverse biological properties. By maintaining the heterogeneity, HSCs maintain the hematopoietic system for whole life, and it seems that it contributes to respond to various stresses. In this study, we focused on mTOR complex and forkhead transcription factor, FOXO, which are essential for survival and cell cycle regulation, and we identified molecules that are regulated by these pathways. Furthermore, by performing CRISPR/Cas9 screening system, we identified multiple functional molecules that contribute to regulate heterogeneity of HSCs and leukemic stem cells.

Free Research Field

幹細胞生物学