Study for stable recruitment of Polycomb-repressive complex-1 on its target loci

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460379
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General medical chemistry
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Isono Kyoichi 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 客員研究員 (90323435)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 発現制御 / ヒストン修飾 / ポリコーム

Outline of Final Research Achievements

Polycomb-repressive complex-1 (PRC1) including Cbx2, Ring1b, Mel18, and Phc2 are a chromatin regulator that plays central roles in stem cell identities. Little is known about the mechanisms of how PRC1 is stably recruited to its target gene loci. Cbx2 has an amino acid sequence typical to a DNA-binding motif AT-hook, suggesting a key player for PRC1 recruitment. Using mouse genetic approach, we here show that Cbx2 AT-hook is responsible for not only stable recruitment of Ring1b to target genes but also PRC1-mediated gene repressions. Our findings would provide new insights into stem cell regulations.

Free Research Field

発生生物学、分子生物学、エピジェネティクス