Proteomic and pathological analyses of arterial aging and aortic dissection, especially focused on expression and posttranslational modification of the proteins

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460432
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: SAWABE Motoji 東京医科歯科大学, 大学院保健衛生学研究科, 教授 (30196331)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 動脈 / 老化 / プロテオーム / 動脈解離症

Outline of Final Research Achievements

Previous studies reported that several proteins produced in vascular smooth muscle cells altered with aging, resulting in the deterioration of vascular function. We performed a proteomic study on the human aortic media with chronological aging, using autopsy samples of the aortic media from elderly, middle-aged, and young subjects. Two proteomic approaches, 2D-DIGE and iTRAQ, were performed. Differentially expressed proteins with aging were categorized into three groups: actin family proteins, extracellular matrix proteins, and antioxidant proteins. A decrease in actin and increase in other actin-related proteins in elderly media were found. The immunohistochemical analysis showed that expressions of antioxidant proteins and oxidation stress-marker proteins were higher in the elderly than in the young. In conclusions, the imbalance between actin and actin-related proteins occur with human aortic aging, and antioxidant proteins were induced by elevations in oxidative stress with aging.

Free Research Field

人体病理学