Application for pathological diagnosis of pleural sarcomatoid mesothelioma based on comprehensive gene expression analysis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460452
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Human pathology
• Research Institution: Hiroshima University
• Principal Investigator: Yukio Takeshima 広島大学, 医歯薬保健学研究院(医), 教授 (70236462)
• Research Collaborator: Amatya V. Jeet 広島大学, 医歯薬保健学研究院(医), 講師 (90403625) ; KUSHITANI KEI 広島大学, 医歯薬保健学研究院(医), 助教 (00508179)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 中皮腫 / 病理診断 / 免疫染色 / 抗体

Outline of Final Research Achievements

Pathological diagnosis of pleural sarcomatoid mesothelioma is difficult, especially differentiating from pulmonary sarcomatoid carcinoma. In this study, We conducted a comprehensive gene expression analysis extracted from formalin-fixed paraffin embedded tissue by GeneChip technology. At the result, MUC4 mRNA expression in mesothelioma was significantly lower than that of carcinoma. The we analyzed MUC4 expression in sarcomatoid mesotheliomas and sarcomatoid carcinomas by immunohistochemistry. As the result, mesotheliomas showed no MUC4 expression, however, approximately 70% cases of sarcomatoid mesotheiomas show positive for MUC4 antibody. From these result, MUC4 might be useful for differential diagnosis between sarcomatoid mesothelioma and pulmonary pleomorphic carcinoma

Free Research Field

病理学