2016 Fiscal Year Final Research Report
Establish of new detection method of gene mutations using cell-free DNA in supernatant fluids
| Project/Area Number |
26460462
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Kurume University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 分子病理学 |
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| Outline of Final Research Achievements |
We examined whether it was possible to detect EGFR mutations in cytology cell-free DNA (ccfDNA) from the supernatant fluids of bronchial and effusion cytology samples. Quantity and fragmentation of ccfDNA in the supernatant fluid and cell damage and CC3 expression in the sediment gradually increased in a time dependent manner in the cell lines. In the 74 clinical samples, the detection of EGFR mutations using ccfDNA showed a high sensitivity in samples with malignant cells. In contrast, EGFR mutations were not found in negative samples. The twenty-two patients of EGFR mutations were detected in 63.6% of ccfDNA from supernatant fluids. EGFR mutations were found in 81.3% (13/16) of malignant cell patients, whereas EGFR mutations were not found in the negative samples. Our results suggest that ccfDNA might help to compensate for the lack of adequate DNA in EGFR mutation analysis, and ccfDNA supernatant fluids can be used to detect EGFR mutation in the same way as cancer cell sediments.
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| Free Research Field |
人体病理学
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