2016 Fiscal Year Final Research Report
Regularatory role of bFGF and microRNA for cardiac remodeling
| Project/Area Number |
26460480
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Toho University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | mircoRNA / bFGF / 心筋間質 / 線維芽細胞 / リモデリング |
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| Outline of Final Research Achievements |
This study shows antifibrotic effects of bFGF injection in hypertensive DS rat hearts carried out using cardiac fibroblast lines harvested from the heart during the LV dilation phase. Specifically, we demonstrated that (1) bFGF inhibited interstitial fibrosis in hypertensive rat heart in vivo; (2) bFGF up-regulated TIMP-1 and down- regulated MMP-9 expression in cardiac fibroblasts (CFs) from hypertensive rat hearts; (3) bFGF also inhibited the expression of collagen I and III in the CFs; (4) real-time PCR array microRNA assays in bFGF treated CFs identified several microRNAs that were up-regulated in the CFs and specifically expressed in interstitial fibrosis of hypertensive DS rat hearts.
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| Free Research Field |
創傷治癒学、組織工学
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