2016 Fiscal Year Final Research Report
Analysis of pathogenesis of virus encephalitis using human iPS 3D culture system
| Project/Area Number |
26460487
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | サイトメガロウイルス / 大脳 / iPS細胞 / neurovascular unit |
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| Outline of Final Research Achievements |
The purpose of this project is to find out new pathogenesis of CMV encephalitis. Unfortunately, we found that it was difficult to establish the 3-D culture of cerebrum from human iPS. We could not achieve the primary goal of this project. But to elucidate the mechanisms of CMV susceptibility in the developing brain, cell tropism and the infectious dynamics of CMV infection were investigated. We evaluated intraventricular and intravascular infections from the perspective of the distribution of CMV and its receptor in the earliest phase of infection. Murine CMV (MCMV) positive cells were colocalized mainly with meninges and choroid plexus or with endothelial cells and pericytes. Therefore, our data demonstrated that the initial distributions of MCMV particles and β1 integrin determine the distinct pattern of infection in the brain in the acute phase. We have published these results in the American Journal of Pathology, Journal of Visualized Experiments, and Pathology International.
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| Free Research Field |
実験病理、人体病理、ウイルス学
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