2016 Fiscal Year Final Research Report
Function of extracellular ADP-ribosyl cyclase BST-1/CD157 in the intestine
| Project/Area Number |
26460499
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Research Collaborator |
HIRANO Hiroyasu 川崎医科大学, 医学部, 大学院生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 腸内細菌叢 / 無菌マウス |
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| Outline of Final Research Achievements |
Bone marrow stromal cell antigen-1(BST-1)/CD157 is a GPI-anchored ectoenzyme having cyclase activity that converts NAD to cyclic ADP-ribose. BST-1 is now appreciated as an entero-neuro-immune regulator. Using the knock-out mice (KO) that lack CD157, CD38 or both, the functions of CD157 in the intestine was examined.The cells expressing CD157 or CD38 locate at distinct regions of the gut system.Decreased severity of DDS-induced colitis in CD157KO indicated that CD157 has a function to promote the colitis. Germ-free CD157KO exhibited the abnormal phenotypes in the cellular dynamics that had not been recognized in the SPF condition. T-RFLP analyses of the feces of double(D)KO revealed a reduced frequency of a certain Operational Taxonomic Unit in the microbiota compared with those of wild type mice. The metabolome analyses of the plasma of DKO revealed the changes in the amount of metabolites in the several pathways.
These data indicate that ADP-ribosyl cyclases regulates the microbiota.
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| Free Research Field |
免疫学
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