2016 Fiscal Year Final Research Report
In vivo analysis of HIV transmission via dendritic cells in humanized mice
| Project/Area Number |
26460500
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOTA Yasuko 東京工科大学, 医療保健学部, 教授 (20182701)
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| Research Collaborator |
IWABUCHI Ryutaro
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ヒト化マウス / 樹状細胞 / HIV / T細胞 / サイトカイン |
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| Outline of Final Research Achievements |
This study was aimed at elucidating the role of dendritic cells (DCs) on HIV-1 transmission in vivo using humanized mice. Because the development of myeloid-lineage immune cells including DCs is poor in conventional humanized mice, introduction of human GM-CSF and Flt3-L into humanized mice was tested to induce DCs. As a result, two myeloid DC subsets, BDCA-1+ MDC1 and BDCA-3+ MDC2, were significantly induced by treatment with these cytokines. When these DC-induced humanized mice and control humanized mice were infected with HIV-1, a significant correlation between the absolute cell number of blood monocytes pre-infection and the plasma viral load at 1 week post-infection was observed. This result suggests that DCs are involved in the efficacy of HIV-1 transmission in vivo at the early phase of infection.
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| Free Research Field |
感染免疫学
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