2016 Fiscal Year Final Research Report
Reaction mechanism analysis and inhibitor screening of helminthic fumarate quinone reductase
| Project/Area Number |
26460504
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Hirosaki University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 蠕虫 / ミトコンドリア / コハク酸脱水素酵素 / ロドキノン / ミトコンドリア呼吸鎖 |
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| Outline of Final Research Achievements |
Echinococcus mitochondrial respiratory complexII contain unusual amino acid at very conserved site, which was Phe instead of common Ala. Same substitution was also found in bacteria Rhodospirillum rubrum. To asses the influence of this amino acid on catalysis, mutaion Ala to Phe and Phe to Ala was introduced into the complex II of Rhodobacter capsulatus and R. rubrum, respectively.
As a result, this mutation read to enhancement of Quinol-Fumarate reductase activities and not much change in Succinate-Quinone reductase activities. This amino acid has some roll on activity adjustment.
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| Free Research Field |
寄生虫学
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