2016 Fiscal Year Final Research Report
Development of a rapid diagnostic test for amebiasis by using fluorescent nanoparticles
| Project/Area Number |
26460516
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Tokai University |
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Principal Investigator |
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| Research Collaborator |
CHENG Xunjia 東海大学, 医学部, 客員教授
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 原虫感染症 / 赤痢アメーバ / 診断 / ナノ材料 |
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| Outline of Final Research Achievements |
Immunochromatographic assay kit using fluorescent silica nanoparticles was developed for rapid serodiagnosis of amebiasis. C-terminal fragment of intermediate subunit from Entamoeba histolytica surface lectin (Igl) was prepared in Escherichia coli and used as antigen. When the kit was evaluated by human monoclonal antibody specific to Igl, the lower detection limit was 100 pg. High sensitivity and high specificity of the kit were demonstrated by evaluation using serum samples from patients.
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| Free Research Field |
寄生原虫学
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