Genome wide identification and characterization of a novel carbamenem-resistance factor in Bacteroides fragilis

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460520
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Gifu University
• Principal Investigator: GOTO Takatsugu 岐阜大学, 生命科学総合研究支援センター, 助教 (80326355)
• Co-Investigator(Renkei-kenkyūsha): MORITA Yuji 愛知学院大学, 薬学部, 准教授 (00454322)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: Bacteroides fragilis / カルバペネム耐性菌 / 中等度耐性株 / ゲノム DNA ライブラリー / MFS トランスポーター / 薬剤耐性機構 / 全ゲノム解析

Outline of Final Research Achievements

Carbamenem-resistant Bacteroides fragilis often makes treatment difficult. We aimed to identify a novel carbamenem-intermediate resistance factor using a genomic DNA library of the B. fragilis GAI92214 strain. We identified a major facilitator superfamily transporter that may contribute slightly to meropenem resistance in the GAI92214 strain. However, we could not fully explain carbamenem-intermediate resistance mechanism from this transporter alone. Thus, we started complete genome sequencing project of the GAI92214 strain. To date, we determined approximately 99% of the genomic sequence encoding about 4723 ORFs.

Free Research Field

細菌学