2016 Fiscal Year Final Research Report
Analysis of cytotoxic T3SS1 effectors of Vibrio parahaemolyticus
| Project/Area Number |
26460526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 腸炎ビブリオ / ３型分泌装置 / エフェクター |
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| Outline of Final Research Achievements |
Vibrio parahaemolyticus, a seafood-borne pathogen, elicits cytotoxicity in a manner dependent on its type III secretion system 1 (T3SS1). In this study, intracellular localization and toxicity of T3SS1-effectors of V. parahaemolyticus were analyzed. Expression of AMPlyation-inactive mutants of VopS in HeLa cells still induced cell morphological changes. Although an N-terminal plasma membrane-localizing domain of VopS alone did not induced cell morphological changes, the deletion of the N-terminal domain in VopS AMPlyation-inactive mutants impaired the toxicity. These results suggested that VopS exhibits AMPlyation-independent toxicity inside host cells, which is required for the localization to the plasma membrane.
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| Free Research Field |
細菌学
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