2016 Fiscal Year Final Research Report
Structure-function analysis of bacterial toxins in tissue destruction to apply their functional domains to regenerative medicine
| Project/Area Number |
26460527
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Okayama University |
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Principal Investigator |
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| Research Collaborator |
SAKON Joshua University of Arkansas・Fulbright college of Arts and Sciences, Chemistry and Biochemistry Department, Professor
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | 細菌毒素 / コラーゲン結合ドメイン / 細胞外マトリックス / 膠原線維 / アンカリング / 再生医療 / 骨新生 / 技術移転 |
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| Outline of Final Research Achievements |
Flesh-eating bacteria produce collagenases to degrade collagen fibrils and to destruct the host tissue. The enzymes consist of a catalytic domain, PKD domain(s) (PKD), and collagen-binding domain(s) (CBD). Since PKD enhanced the binding of CBD to insoluble collagen, PKD-CBD region derived from a collagenase was used to anchor growth factors to collagenous matrix, e.g. high-density collagen sheet or demineralized bone matrix. These composite materials were utilized to induce osteogenesis at bone defect sites. 3D-structure of PKD was determined by X-ray crystallography, which gave an insight into the molecular basis of the collagen anchoring. Furthermore, dimeric CBD was shown to be an appropriate anchor for a synthetic collagenous matrix.
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| Free Research Field |
基礎医学・細菌学
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