2016 Fiscal Year Final Research Report
Role of the sugar metabolism of resident microbiota in the gut homeostasis
| Project/Area Number |
26460530
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 腸管内常在菌 / Bacteroides / Clostridium difficile / 毒素 / 溶菌 / 糖代謝 |
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| Outline of Final Research Achievements |
Inhibitory effect of Bacteroides thetaiotaomicron (BT) on the toxinogenicity of Clostridium difficile was evaluated in the co-infection model using BALB/c germ-free mice. The fecal Toxin A level in the mice infected with wild type BT and CD was lower than those in the mice colonized with CD and PS6- or polysaccharide transport-deficient BT. This result indicates that the BT inhibition on CD toxin production is mediated by BT polysaccharides. Cytotoxicity assay to HT-29 cells with the fecal supernatant showed the similar results as described above. In addition, the number of CD cell stained in Gram-positive was higher in the feces from the mice infected with wild BT and CD than the mice in other groups. These results indicate that the BT inhibition on CD toxin production is due to the suppression of CD autolysis by BT polysaccharides.
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| Free Research Field |
微生物学
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