Functional analysis and epidemiological survey of a novel Stx2 phage isolated from the food-poisoning outbreak

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460544
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bacteriology (including mycology)
• Research Institution: Toyama Institute of Health
• Principal Investigator: WATAHIKI Masanori 富山県衛生研究所, 細菌部, 部長 (20372104)
• Co-Investigator(Renkei-kenkyūsha): ISOBE Junko 富山県衛生研究所, 細菌部, 副主幹研究員 (10421893) ; KIMATA Keiko 富山県衛生研究所, 細菌部, 主任研究員 (50360805)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 食中毒 / 腸管出血性大腸菌 / EHEC O111 / EHEC O157 / Stx2ファージ / Ⅱ型制限修飾酵素 / 遺伝子破壊 / ファージ疫学

Outline of Final Research Achievements

Enterohemorrhagic Escherichia coli (EHEC) O111 and O157 were detected in the food-poisoning outbreak case in 2011, and Stx2 phages carrying a type II restriction(R)-modification (M) gene were also detected in some severe patient feces and the lysate from EHECO111. To elucidate relation of the function of R gene and some pathogenicity of the outbreak, Stx2 phage with R gene disrupted was prepared for functional analysis of the gene. It was suggested that R gene promotes Stx2 production in the presence of EHEC O111 and O157. Therefore, Stx2 phage with RM gene is considered to be a key player in the outbreak. Finally, in surveillance of Stx2 phage contamination in some food specimens, Stx2 phage was not detected.

Free Research Field

基礎医学　細菌学　遺伝・ゲノム情報