2016 Fiscal Year Final Research Report
Recovery of functional suspend of T-cells membrane lipid raft caused by HIV using anti-myristoylation diabody.
| Project/Area Number |
26460551
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | ミリストイル化 / 抗体 / HIV / AIDS / membrane lipid raft / 抗体ライブラリ |
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| Outline of Final Research Achievements |
Addition of original anti-myristoylation antibody reduced precipitation of myristoylated HIV-Nef in the presence of lipid micelle, and this result indicates inhibitory effect of the antibody restraining interaction between myristoylated molecules and lipid membranes.
Next, anti HIV-Nef antibody with capacities of high specificity and low dissociation constant (10^-9) was developed by original antibody library technique.
A diabody was constructed by a fusion of the antibody with the anti- myristoylation antibody. Addition of the diabody preliminary reduced precipitation of myristoylated HIV-Nef in the presence of lipid micelle again. The remaining subject is specificity of the diabody to myristoylated proteins in the cells.
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| Free Research Field |
分子生物学
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