2016 Fiscal Year Final Research Report
Development of assay utilizing DNA aptamer and applying to a clinical examination
| Project/Area Number |
26460656
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | アプタマー / オキシトシン / アスペルガー / 臨床分析化学 |
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| Outline of Final Research Achievements |
We studied to development assay utilizing aptamer for oxytocin (OXT), was shown to play important key roles in social interactions and social behavior and a series of studies investigated the relationship between autism spectrum disorder (ASD) and OXT, and applying to a clinical examination by using that assay.
As a result of SELEX, the aptamers (ssDNA) for OXT prepared in random ssDNA BANK and sequence of those were high homologous sequence. Therefore, we suggested that aptamer for OXT was selected from many ssDNA containing random sequence. Moreover, we made a measurement of OXT in Japanese human plasma on this study by using immunoassay for OXT. As a result, the average levels of males and females were 30 and 25 pg/mL respectively, here.
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| Free Research Field |
医歯薬学
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