Phosphorylation of ATBF1 and its intracellular localization in malignant mestothelioma - investigation of the association with malignant behavior

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460660
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kanazawa Medical University
• Principal Investigator: MINATO Hiroshi 金沢医科大学, 医学部, 教授 (10293367)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 悪性中皮腫 / ATBF1 / 癌抑制遺伝子 / 培養細胞 / 細胞内局在 / 核細胞質移行 / 遺伝子ノックアウト / リン酸化

Outline of Final Research Achievements

We cultured human malignant mesothelioma cell lines (NCI-H 2452, MSTO-211 H, NCI-H 28) and benign mesothelial cell line (Met-5 A) to explore the role of ATBF 1 in malignant mesothelioma. ATBF1 knockout strain was prepared from Met-5A strain using a CRISPR method. Luc gene was inserted into these Met-5A sub-strains and MSTO-211 H, NCI-H 28 strain and transplanted into the abdominal cavity of nude mice. By observing the change in the amount of luminescence, tumor growth was successfully confirmed intraabdominal cavity in MSTO-211H, whereas tumor growth could not be confirmed in Met-5A, NCI-H28 strain after about 2 weeks. Currently, knockout of ATBF1 alone may not cause tumorigenesis.

Free Research Field

病理学