2016 Fiscal Year Final Research Report
Mechanism of production, secretion and hypertension in the endogenous digitalis like factors using mass spectrometry immunoassay
| Project/Area Number |
26460662
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Kansai Medical University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | 内因性ジギタリス / 質量分析イムノアッセイ / 高血圧 / 生理活性物質 / 質量分析 |
|---|
| Outline of Final Research Achievements |
This research is a part of patho-physiological study of endogenous digitalis-like factors (EDLFs). We have marinobufagin, telocinobufagin, and their related compounds, maronobufotoxin and telicinobufotoxin. Moreover, we reported they are existed in human plasma. EDLFs have low monocular compounds with steroid ring. Therefore their analytic methods have only some techniques. Recently MISA, which has been interested by same field’s researchers, can be a technique for high sensitive qualitative as well as quantitative analysis in lesser sample volunme. We tried MISA was used more easily and high specific pretreatment of our ELISA instead of C18 seppak columns. Our each antibodies was absorbed in MISA tubes, and then samples was added in MISA tube. In this tube, we tried to extract only target substances.
Unfortunately, the adequate antigen‐antibody reactionin was not achieved in this study.
|
| Free Research Field |
医歯薬
|
