The expression and localization of RNase and RNase inhibitor in blood cells and vascular endothelial cells in homeostasis of the vascular system

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460668
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Koyama Takatoshi 東京医科歯科大学, 大学院保健衛生学研究科, 准教授 (20234916)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: RNase / RNase inhibitor (RI) / 血管内皮細胞 / 血小板

Outline of Final Research Achievements

RNA may be released from vascular cells including endothelial cells in the event of injury and in vascular disease. RNase activity in the supernatant from the human umbilical vein endothelial cell line EAhy926 cells was 50 times than in blood cells (after 60 min). RNase 1 mRNA and protein expression in EAhy926 cells was highest among the cells examined. However, RNase inhibitor (RI) mRNA and protein expression was similar in most cell types examined. Furthermore, we observed that RNase 1 and von Willebrand factor were partially colocalized in EAhy926 cells and platelets. In conclusion, we propose that high RNase activity is ordinarily released from endothelial cells to support anticoagulation in the vasculature. On the other hand, platelets and leukocytes within thrombi at sites of vascular injury show very low RNase activity, which may support hemostatic thrombus formation. However, activated platelets and leukocytes may accelerate pathologic thrombus formation.

Free Research Field

血液凝固調節