2016 Fiscal Year Final Research Report
Genetic analysis for inherited arrhythmia and functional study using zebrafish model
| Project/Area Number |
26460670
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
SEKINE Akihiro 京都大学, 医学系研究科, 教授 (30425631)
AIBA Takeshi 独立行政法人国立循環器病研究センター, 循環器内科, 医長 (40574348)
|
|---|
| Research Collaborator |
TSUDA Toyonobu
TANAKA Yoshihiro
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Keywords | ゼブラフィッシュ / 次世代シークエンス / モルフォリノ / Crispr/Cas9システム / 遺伝子改変ゼブラフィッシュ |
|---|
| Outline of Final Research Achievements |
We performed genetic analysis using next generation sequencing for patients with inherited arrhythmia.We performed electrophysiological study for detected gene mutations in ion channel genes using patch clamp methods. We evaluated mutations in KCNH2 gene using zebrafish embryo. Knockdown or CRISPR-mediated deletions of the human LMNA ortholog, lmna in zebrafish recapitulated the electrophysiological phenotypes of human early-onset bradycardia.
|
| Free Research Field |
循環器内科
|
