Development of a novel platelet-biogenesis marker, BDNF

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460671
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: University of Yamanashi
• Principal Investigator: OZAKI Yukio 山梨大学, 総合研究部, 医学研究員 (30134539)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 血小板 / 巨核球 / BDNF

Outline of Final Research Achievements

Previously, we reported that brain-derived neurotrophic factor (BDNF) had a novel function which involved in a megakaryocyte differentiation using human megakaryocyte cell-line model, MEG-01. In this study, we investigated whether BDNF affected normal primary megakaryocyte development in mice. BDNF accelerated clonal expansion of primary megakaryocyte progenitor in vitro. On the other hand, megakaryocyte maturation was not promoted by BDNF. These results suggested that BDNF had a potential as a Meg-CSF for primary megakaryocyte. However, BDNF did not show the physiological function to promote a platelet recovery in transient thrombocytopenic mouse model with anti-platelet antibody. Last year, it was reported that mouse megakaryocytes did not produce BDNF, whereas human megakaryocytes produce BDNF and platelets stored abundant BDNF in alpha-granules. To further investigate a BDFN pathophysiological function for in vivo megakaryopoiesis, an alternative in vivo model should be demanded.

Free Research Field

医歯薬学