Detection and pathological analyses of specific antibody in cat scratch disease by highly-sensitive ELISA using Japanese-derived strain

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460678
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Yamaguchi University
• Principal Investigator: TSUNEOKA Hidehiro 山口大学, 医学(系)研究科(研究院), 教授 (40437629)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: Bartonella henselae / 猫ひっかき病 / ELISA / CHAPS

Outline of Final Research Achievements

The measurement of anti-Bartonella henselae IgG by highly-sensitive ELISA requires highly-specific purification of antigen. To obtain this antigen, it is needed 1) to culture Bartonella henserae in double-phase culture media (Heart infusion agar medium with 5％rabbit blood and D-MEM liquid medium), 2) to put the liquid in 0.1％ CHAPS (ampholytic surfactant) at 37℃ to extract insoluble components, which is to be put in 0.5% CHAPS for the second time to extract soluble components, and 3) to purify the protein in the soluble components using 245-310mMNaCL by DEAE-Sepharose chromatography. This purified solution contains highly-specific protein components and therefore, clinical implication of this protein as an antigen is greatly expected in the measurement of anti-Bartonella henserae IgG by ELISA.

Free Research Field

臨床微生物学