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2017 Fiscal Year Final Research Report

Development of novel classification of mycobacteria by whole genome analysis and mass spectrometry

Research Project

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Project/Area Number 26460686
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory medicine
Research InstitutionKyorin University

Principal Investigator

Matsushima Satsuki  杏林大学, 医学部, 実験助手 (80231596)

Co-Investigator(Kenkyū-buntansha) 渡邊 卓  杏林大学, 医学部, 教授 (00191768)
大塚 弘毅  杏林大学, 医学部, 学内講師 (70439165)
大西 宏明  杏林大学, 医学部, 教授 (80291326)
Project Period (FY) 2014-04-01 – 2018-03-31
Keywords非結核性抗酸菌 / 全ゲノム解析 / 質量分析
Outline of Final Research Achievements

We performed whole genome analysis and mass spectrometry of Mycobacterium kyorinense and its related mycobacterium species. M. kyorinense and its related species demonstrated common features in several drug-resistance related genes including rpoB, inhA, katG, ahpC, and embB, but full genome data were not available in these mycobacteria due to technical issues. We successfully performed mass spectrometry for these species, but they were difficult to distinguish by mass spectrometry, probably due to their high similarity of 16S rRNA between species.

Free Research Field

臨床検査医学

URL: 

Published: 2019-03-29  

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