Novel apoptotic mechanism induced by Cables

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460690
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kobe Tokiwa University
• Principal Investigator: Sakamoto Hideo 神戸常盤大学, 保健科学部, 教授 (30225817)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: Cables / アポトーシス / プロテインキナーゼC

Outline of Final Research Achievements

Previously, I reported that loss of Cables expression is observed in several tumors and high expression of Cables induced apoptosis. From these findings, I hypothesized that Cables is getting increased in early stage of tumor and cause cell death to prevent tumor. Evidence of hyper methylation of the Cables promoter was also observed in several tumors.
These findings suggested that Cables expression in tumor may be regulated the promoter activity. To evaluate the Cables promoter regulation, I evaluated Cables promoter activity in endometrial tumor derived culture cell lines. Cells were stimulated by Progesterone, Phorbol12-Myristate13-acetate (PMA) or Forskolin. As result, Cables promoter activity has increased in one of cell lines by PMA which is an activator of protein kinase C (PKC). This result suggest that Cables promoter regulation is depends on the cell type. The PKC regulation to Cables promoter is not clear, however it is one of strong regulator of Cables promoter activity.

Free Research Field

病態検査