METAGENOMICS OF MICROBIAL COMMUNITIES IN GALLBLADDER BILE FROM PATIENTS WITH GALLBLADDER CANCER OR CHOLELITHIASIS

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460812
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: Niigata University (2015-2016); Niigata University of Health and Welfare (2014)
• Principal Investigator: TSUCHIYA Yasuo 新潟大学, 医歯学総合研究科, 客員研究員 (60334679)
• Research Collaborator: LOZA Ernest ボリビア・日本消化器センター ; VILLA-GOMEZ Guido ボリビア・日本消化器センター ; TRUJILLO Carlos ボリビア・日本消化器センター ; BAEZ Sergio ソテロ デル リオ病院 ; OKUDA Kayo タカラバイオ株式会社 ; NOZAKI Ryoko 臨床検査技師
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 胆嚢癌 / 胆石症 / 胆嚢胆汁 / 次世代シーケンサー / メタゲノム解析 / 16S rRNA

Outline of Final Research Achievements

Salmonella typhi and Helicobacter infections have been shown to increase risk of gallbladder cancer (GBC), but findings have been inconsistent. Other bacterial infections may also be associated with GBC. We aimed to investigate the microbial communities in gallbladder bile of patients with GBC and cholelithiasis (CL). DNA was extracted from bile in seven GBC patients and 30 CL patients, and the V3-V4 region of 16S rRNA was amplified. The sequencing results were compared with the 16S database. DNA was detected in the bile of three GBC (42.9%) and four CL patients (13.3%). As the predominant species, Fusobacterium nucleatum, Escherichia coli, and Enterobacter sp. were detected in bile from GBC patients. Those in bile from CL patients were Escherichia coli, Salmonella sp., and Enterococcus gallinarum. Whether the three bacteria detected in bile from GBC patients would associated with the development of GBC just like colorectal cancer remains unknown.

Free Research Field

医歯薬学