Cancer risk assessment based on oxidative and nitrative DNA damage in murine lung exposed to Asian sand dust

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460813
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Hygiene and public health
• Research Institution: Suzuka University of Medical Science
• Principal Investigator: OHNISHI Shiho 鈴鹿医療科学大学, 薬学部, 助教 (80511914)
• Co-Investigator(Renkei-kenkyūsha): KAWANISHI Shosuke 鈴鹿医療科学大学, 薬学部, 教授 (10025637)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: 環境保健 / がん予防 / 炎症 / DNA損傷 / 黄砂

Outline of Final Research Achievements

Particulate matter in air pollution is estimated to carcinogenic to humans by the International Agency for Research on Cancer. In this study, we found that intranasal exposure to Asian sand dust caused inflammation in murine lung. We demonstrated that 8-oxodG, oxidative DNA damage, and 8-nitroguanine, nitrative DNA damage, were increased in lung tissues of mice exposed to Asian sand dust, by histochemical analysis. The proteomic analysis revealed several tens of up-regulated or down-regulated proteins by Asian sand dust. One of up-regulated proteins was identified to the protein that was reported to have relevance to anti-oxidation and anti-tumor. One of down-regulated proteins was identified to the protein that was reported the possibility of anti-tumor and decrease in human lung tumor. These suggest that Asian sand dust induces oxidative and nitrative DNA damage in the lung via inflammation.

Free Research Field

衛生学