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2016 Fiscal Year Final Research Report

Individual identification of transferred cells from the stain samples.

Research Project

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Project/Area Number 26460890
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Legal medicine
Research InstitutionNihon University

Principal Investigator

MARUYAMA Sayaka  日本大学, 歯学部, 助教 (30366190)

Project Period (FY) 2014-04-01 – 2017-03-31
Keywords個人識別 / 法医学 / トランスファー細胞 / WGA / mtDNA多型 / 常染色体DNA多型 / Y染色体DNA多型
Outline of Final Research Achievements

We collected transfer cells and used them to perform whole genome amplification (WGA) with the REPLI-g Single Cell Kit and purified it of the genomic DNA.
As for the cell count, it is thought that 15-20 are necessary because a judgment of all inspection of mtDNA, autosomal STR and Y-STR is enabled.

Free Research Field

法医学

URL: 

Published: 2018-03-22  

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