Identification of gastric cancer risk factors after H. pylori eradication by evaluating exhaustive genetic analysis with using next-generation sequencing.

2017 Fiscal Year Final Research Report

• Project/Area Number: 26460942
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Oita University
• Principal Investigator: Kodama Masaaki 大分大学, 福祉健康科学部, 教授 (20332893)
• Co-Investigator(Kenkyū-buntansha): 村上 和成 大分大学, 医学部, 教授 (00239485) ; 沖本 忠義 大分大学, 医学部, 講師 (90381037)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: Helicobacter pylori / 除菌後胃癌 / 癌遺伝子 / 次世代シーケンサー解析 / TP53 / PDGFRA

Outline of Final Research Achievements

Exhaustive genetic characteristics of gastric cancer (GC) after H. pylori eradication was elucidated with using next-generation sequencing. 20 subjects (5: well differentiated GC before eradication, 5: well differentiated GC after eradication, 6: undifferentiated GC before eradication, 4: undifferentiated after eradication) were evaluated with using the Ion Ampliseq V2 Cancer Hot spot panel (Life Technologies). Genetic mutation was found in more than 80% subjects in oncogene APC, CSF1R, FGFR3, FLT3, KDR, PDGFRA, RET, STK11, TP53. Number of hotspot mutation of well differentiated GC group showed significantly few than the other group. In addition, only well-differentiated GC after eradication group did not detect the hotspot mutation of PDGFRA at all. GC after H. pylori eradication had different characteristics. Especially, the present result showed that well differentiated GC after eradication was considered to have the different carcinogenic mechanism genetically.

Free Research Field

消化器内科