Molecular mechanism elucidation of gastric and intestinal differentiation and development of differentiation-inducing therapy against stem cell

2017 Fiscal Year Final Research Report

• Project/Area Number: 26460945
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Nagoya City University
• Principal Investigator: Mizoshita Tsutomu 名古屋市立大学, 大学院医学研究科, 講師 (40347414)
• Co-Investigator(Kenkyū-buntansha): 谷田 諭史 名古屋市立大学, 大学院医学研究科, 講師 (30528782) ; 城 卓志 名古屋市立大学, 大学院医学研究科, 教授 (30231369)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: in vitro三次元培養腺管 / 胃幹細胞

Outline of Final Research Achievements

The analyses in long-term three-dimensional method for primary mouse glandular stomach culture have shown that the gastric mesenchymal myofibroblast (GMF) is important for the differentiation and proliferation of gastric epithelial cell. Growth arrest specific 1（GAS1）expression is higher in GMF, and Homeobox C8（HoxC8）、Notch 1、SRY-box containing gene 10（Sox10） are higher in intestinal mesenchymal myofibroblast, suggesting that these factors play the key roles in the gastric and intestinal differentiation by epithelial-mesenchymal interactions. We consider that the ectopic MUC5AC expression in the intestinal epithelial cells may be a predictive factor of recurrence or remission in the cases of Crohn's disease and intestinal Behçet's disease.

Free Research Field

消化器内科