Analysis for viral factors and host factors influencing hepatitis C therapeutic effect.

2016 Fiscal Year Final Research Report

• Project/Area Number: 26460987
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Itsui Yasuhiro 東京医科歯科大学, 医学部附属病院, 講師 (20401341)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Keywords: インターフェロン誘導遺伝子 / C型肝炎ウイルス / GBP-1 / NS5B

Outline of Final Research Achievements

We have focused on interferon stimulated genes (ISGs), which were involved in efficacy of interferon (IFN)-based therapy for chronic hepatitis C, and investigated regulatory effects of ISG on viral replication in hepatitis C virus (HCV). (1) We have produced newly established cell lines, HCV subgenomic replicon (Huh7/Rep-Feo), which were in-vitro models simulating cellular autonomous replication of HCV genomic RNA. (2) We showed that GBP-1, IFI-6-16, and IFI-27 had directly inhibited subgenomic replication and virus production of HCV. (3) Investigation of molecular interaction of the ISGs with HCV proteins showed that GBP-1 bound HCV-NS5B directly. A protein truncation assay showed that the guanine binding domain of GBP-1 and the finger domain of NS5B were involved in the interaction.

Free Research Field

肝臓病学