2016 Fiscal Year Final Research Report
Molecular mechanism of hepatitis C virus-induced lipid droplet formation
| Project/Area Number |
26461003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kobe University |
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Principal Investigator |
SHOJI Ikuo 神戸大学, 医学研究科, 教授 (40356241)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Keywords | HCV / 脂肪滴 / NS3/4A プロテアーゼ / 脂肪化 |
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| Outline of Final Research Achievements |
(1) SPG20 was cleaved in HCVcc-infected Huh-7.5 cells. (2) Transient expression of HCV NS3/4A resulted in cleavage of SPG20. (3) Inactive mutant NS3/4A did not cleave SPG20. (4) NS3/4A inhibitor inhibited cleavage of SPG20, whereas NS5A inhibitor did not inhibit cleavage of SPG20. (5) Treatment of the HCVcc-infected Huh-7.5 cells decreased the size of lipid droplets. Taken together, our results suggest that HCV NS3/4A cleaves SPG20 thereby promoting enlargement of lipid droplet in HCV-infected cells.
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| Free Research Field |
ウイルス学
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