Development of hybrid-conditioning methods to salvage myocardium and elucidation of HSP mediated cardioprotection

2017 Fiscal Year Final Research Report

• Project/Area Number: 26461052
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Asahikawa Medical College
• Principal Investigator: Takeuchi Toshiharu 旭川医科大学, 医学部, 講師 (10372278)
• Co-Investigator(Kenkyū-buntansha): 蓑島 暁帆 旭川医科大学, 医学部, 特任助教 (90645962) ; 長谷部 直幸 旭川医科大学, 医学部, 教授 (30192272)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 急性心筋梗塞 / 再灌流障害 / 熱ショック蛋白 / ポストコンディショニング / リモート・コンディショニング / ハイブリッド・コンディショニング

Outline of Final Research Achievements

The first acute myocardial infarction ｐatients were randomly assigned to the control group and the hybrid-conditioning group. In the hybrid-conditioning group, three cycles of remote preconditioning were repeated, with the lower limb being pressurized for 5 minutes and depressurized for 5 minutes. After remote preconditioning, postconditioning was performed 4 cycles of 60s balloon inflation and 30s deflation, and finally the appropriate sized stent was placed. In both groups, blood was collected over time with time before and after reperfusion treatment, and the expression level of HSP72 in the separated leukocytes was quantitatively evaluated. Hybrid-conditioning method was shown to increase the expression of HSP 72, resulting in suppression of reperfusion injury.

Free Research Field

循環器内科学