Reproduction of injured myocardium and introduction of reverse remodeling by implantation of Kv1.3 transfected fibroblast.

2017 Fiscal Year Final Research Report

• Project/Area Number: 26461085
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cardiovascular medicine
• Research Institution: Kitasato University
• Principal Investigator: NIWANO SHINICHI 北里大学, 医学部, 准教授 (70282978)
• Co-Investigator(Kenkyū-buntansha): 庭野 裕恵 北里大学, 医学部, 非常勤講師 (00293233)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Keywords: 心不全 / イオンチャンネル / 炎症 / 再生医療 / リモデリング

Outline of Final Research Achievements

As the in-vivo heart failure model, isoproterenol injection model was employed with dose of 80mg/kg/day for 2 days. Histological evaluation showed myocardial hypertrophy, degeneration and interstitial proliferation, and the electrophysiological evaluation found prolongation of effective refractory period (mean 52%) and MAP duration (120% MAP-20 and 56% MAP-90). Early step of inflammation was considered to play a role in these changes because increase of CTGF, TGF-beta, MCP-1, and IL-2 were found. By re-design of plasmid, transfection of Kv1.3 to the fibroblast was improved, but the reproducibility was still limited.

Free Research Field

循環器内科学